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KMID : 0545120090190040378
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Journal of Microbiology and Biotechnology
2009 Volume.19 No. 4 p.378 ~ p.386
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Bioprocess Development for Production of Alkaline Protease by Bacillus pseudofirmus Mn6 Through Statistical Experimental Designs
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Abdel-Fattah Y.R.
El-Enshasy H.A.
Soliman N.A.
El-Gendi H.
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Abstract
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A sequential optimization strategy, based on statistical experimental designs, is employed to enhance the production of alkaline protease by a Bacillus pseudofirmus local isolate. To screen the bioprocess parameters significantly influencing the alkaline protease activity, a 2-level Plackett-Burman design was applied. Among 15 variables tested, the pH, peptone, and incubation time were selected based on their high positive significant effect on the protease activity. A nearoptimum medium formulation was then obtained that increased the protease yield by more than 5-fold. Thereafter, the response surface methodology (RSM) was adopted to acquire the best process conditions among the selected variables, where a 3-level Box-Behnken design was utilized to create a polynomial quadratic model correlating the relationship between the three variables and the protease activity. The optimal combination of the major medium constituents for alkaline protease production, evaluated using
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KEYWORD
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Haloalkaliphiles, optimization of alkaline protease, statistical experimental design
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